Try crusade "fast" at room temperature: Immediate Spin
essentially serves to confirm the AB0 compatibility between recipient and donor. Should always be done, especially if you do not routinely double check .(....)
Technique:
> mix 3 drops of serum / plasma of the recipient + 1 drop of red blood cells donor in suspension at 3-4% in saline.
> read for agglutination.
Even this test may be performed in test tubes (centrifugation and read out as above) or slide / plate, slide, however, this survey is less reliable and requires more time and a closer reading eventually with the aid of a microscope at low magnification.
Interpretation:
Absence of agglutination: the recipient and the donor are confirmed compatible with respect to the AB0 group.
· Presence of agglutination: mean incompatibility (ABO or other antibodies that may be cold or not clinically significant) NOT transfuse, gruppaggio check a possible error in the direct evidence and assess the possible presence of cold auto-antibodies (see Indirect determination ABO).
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